Translational_Unit

Part:BBa_M36002:Design

Designed by: Elliott Lui and Jesse Palmer   Group: Stanford BIOE44 - S11   (2011-04-28)

gluc1phos adenylyltrans (with 5'UTR & term)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 618
    Illegal EcoRI site found at 1115
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 618
    Illegal EcoRI site found at 1115
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 618
    Illegal EcoRI site found at 1115
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 618
    Illegal EcoRI site found at 1115
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 618
    Illegal EcoRI site found at 1115
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This mutation results in greater glycogen production compared to WT.


Source

The sequence provided here is from the CL1136 strain, with an Arg67Cys mutation in the glgC gene.

References

Meyer CR, Ghosh P, Remy E, Preiss J. Cloning, expression, and nucleotide sequence of a mutant glgC gene from Escherichia coli B. J Bacteriol. 1992 Jul;174(13):4509–4512

http://biocyc.org/ECOLI/NEW-IMAGE?type=PATHWAY&object=GLYCOGENSYNTH-PWY&detail-level=3